PrimePCR™ Probe Assay: CT030687.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CT030687.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CT030687.1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043925
Assay Design:   exonic
Chromosome Location:   12:88249911-88250051question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000078079 ENSMUST00000168382 ENSMUST00000110152 ENSMUST00000164838 ENSMUST00000164517 ENSMUST00000181313 ENSMUST00000110149 ENSMUST00000110147 ENSMUST00000095510 ENSMUST00000180053 ENSMUST00000110148 ENSMUST00000180285 ENSMUST00000178301 ENSMUST00000178852 ENSMUST00000177720 ENSMUST00000179468 ENSMUST00000101168 ENSMUST00000177747 ENSMUST00000091715

Gene Information

Description Not Available

Gene Symbol:   CT030687.1
Gene Name:   Eukaryotic translation initiation factor 1A-like 2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095799
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 38.290000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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