PrimePCR™ Probe Assay: Dynlt1f, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043913
Assay Design:   exonic
Chromosome Location:   17:6655774-6655867question
Amplicon Length:   64
Splice Variants Targeted:   ENSMUST00000106072 ENSMUST00000071108 ENSMUST00000129190 ENSMUST00000068168 ENSMUST00000113987 ENSMUST00000025468 ENSMUST00000135688 ENSMUST00000047865 ENSMUST00000152071 ENSMUST00000142850 ENSMUST00000133193 ENSMUST00000153478 ENSMUST00000062446 ENSMUST00000102812 ENSMUST00000075372 ENSMUST00000080658 ENSMUST00000114674 ENSMUST00000043775 ENSMUST00000030795 ENSMUST00000169415 ENSMUST00000179569 ENSMUST00000092966 ENSMUST00000179554 ENSMUST00000063857 ENSMUST00000030269 ENSMUST00000164853 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Dynlt1f
Gene Name:   Dynein light chain Tctex-type 1F
Aliases:   100040631, Dynlt1e
RefSeq:   NC_000083.6 NT_039636.8
Ensembl:   ENSMUSG00000095677
Entrez:   100040531
UniGene:   Mm.458102
Chromosome Mapping:   17 A1|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 37.580000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download