PrimePCR™ Probe Assay: AC239677.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043899
Assay Design:   exonic
Chromosome Location:   5:25954206-25954319question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000022075 ENSMUST00000167847 ENSMUST00000162387 ENSMUST00000168875 ENSMUST00000072286 ENSMUST00000079447 ENSMUST00000095004 ENSMUST00000094946 ENSMUST00000063524 ENSMUST00000088236 ENSMUST00000074148 ENSMUST00000053257 ENSMUST00000155721 ENSMUST00000166086 ENSMUST00000076099 ENSMUST00000088625 ENSMUST00000170064 ENSMUST00000124677 ENSMUST00000035980 ENSMUST00000159973 ENSMUST00000096953 ENSMUST00000167908 ENSMUST00000095006 ENSMUST00000092696 ENSMUST00000095005 ENSMUST00000179506 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC239677.1
Gene Name:   Not available
Aliases:   Not Available
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000095550
Entrez:   100862359
UniGene:   Not Available
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 37.770000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download