PrimePCR™ Probe Assay: AC195646.2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best specificity

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043894
Assay Design:   exonic
Chromosome Location:   Y:25995644-25995755question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000177666 ENSMUST00000179829 ENSMUST00000178181 ENSMUST00000179949 ENSMUST00000178149 ENSMUST00000179922 ENSMUST00000178903 ENSMUST00000178326 ENSMUST00000181549 ENSMUST00000178889 ENSMUST00000115496 ENSMUST00000171947 ENSMUST00000181205 ENSMUST00000181163 ENSMUST00000179070 ENSMUST00000181357

Gene Information

Description Not Available

Gene Symbol:   AC195646.2
Gene Name:   Uncharacterized protein LOC100039614
Aliases:   Not Available
RefSeq:   NC_000087.7 NT_187045.1
Ensembl:   ENSMUSG00000095439
Entrez:   100862062
UniGene:   Not Available
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 42.510000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download