PrimePCR™ Probe Assay: CT573034.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043864
Assay Design:   exonic
Chromosome Location:   13:62371814-62371912question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000122450 ENSMUST00000113729 ENSMUST00000170101 ENSMUST00000034259 ENSMUST00000105315 ENSMUST00000105316 ENSMUST00000099439 ENSMUST00000076281 ENSMUST00000105313 ENSMUST00000099449 ENSMUST00000107989 ENSMUST00000076195 ENSMUST00000101547 ENSMUST00000091563 ENSMUST00000167516 ENSMUST00000037372 ENSMUST00000089217 ENSMUST00000001989 ENSMUST00000025645 ENSMUST00000120524 ENSMUST00000130783 ENSMUST00000144085 ENSMUST00000048892 ENSMUST00000105718 ENSMUST00000135798 ENSMUST00000112547 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   CT573034.1
Gene Name:   Uncharacterized protein LOC100041979
Aliases:   Not Available
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000094942
Entrez:   100041979
UniGene:   Not Available
Chromosome Mapping:   13 B3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 40.470000
Efficiency 84

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download