PrimePCR™ Probe Assay: Vmn1r121, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043844
Assay Design:   exonic
Chromosome Location:   7:21097598-21097726question
Amplicon Length:   99
Splice Variants Targeted:   ENSMUST00000079099 ENSMUST00000098747 ENSMUST00000169774 ENSMUST00000172989 ENSMUST00000164245 ENSMUST00000098744 ENSMUST00000173723 ENSMUST00000168794 ENSMUST00000098737 ENSMUST00000166937 ENSMUST00000164683 ENSMUST00000105197 ENSMUST00000168398 ENSMUST00000165961 ENSMUST00000172863 ENSMUST00000105195 ENSMUST00000173484 ENSMUST00000098722 ENSMUST00000163096 ENSMUST00000172551 ENSMUST00000165916 ENSMUST00000098720 ENSMUST00000174643 ENSMUST00000174451 ENSMUST00000168392

Gene Information

Description Not Available

Gene Symbol:   Vmn1r121
Gene Name:   Vomeronasal 1 receptor 121
Aliases:   EG667240, Gm8533
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000094680
Entrez:   667240
UniGene:   Mm.480259
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 39.630000
Efficiency 85

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download