PrimePCR™ Probe Assay: AL844494.1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043798
Assay Design:   exonic
Chromosome Location:   4:42320600-42320734question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000115912 ENSMUST00000053094 ENSMUST00000120223 ENSMUST00000097655 ENSMUST00000035211 ENSMUST00000162886 ENSMUST00000108026 ENSMUST00000178192 ENSMUST00000180201 ENSMUST00000107979 ENSMUST00000096241 ENSMUST00000018632 ENSMUST00000170942

Gene Information

Description Not Available

Gene Symbol:   AL844494.1
Gene Name:   Protein FAM205A
Aliases:   Not Available
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000093996
Entrez:   100862261
UniGene:   Not Available
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 37.210000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download