PrimePCR™ Probe Assay: Vmn1r120, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best negative control

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Vmn1r120, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Vmn1r120, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043796
Assay Design:   exonic
Chromosome Location:   7:21052867-21053016question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000165330 ENSMUST00000079099 ENSMUST00000164526 ENSMUST00000168984 ENSMUST00000169774 ENSMUST00000172989 ENSMUST00000105209 ENSMUST00000177815 ENSMUST00000164245 ENSMUST00000163461 ENSMUST00000169689 ENSMUST00000173886 ENSMUST00000174364 ENSMUST00000179079 ENSMUST00000179511 ENSMUST00000174538 ENSMUST00000173723 ENSMUST00000168794 ENSMUST00000164288 ENSMUST00000166937 ENSMUST00000164683 ENSMUST00000105202 ENSMUST00000164409 ENSMUST00000166948 ENSMUST00000105200 ENSMUST00000098739 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn1r120
Gene Name:   Vomeronasal 1 receptor 120
Aliases:   EG435953, Gm5730
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000093986
Entrez:   435953
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.986400
y-intercept 36.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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