PrimePCR™ Probe Assay: Higd1c, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043772
Assay Design:   exonic
Chromosome Location:   15:100374893-100374996question
Amplicon Length:   74
Splice Variants Targeted:   ENSMUST00000122065 ENSMUST00000072899 ENSMUST00000006444 ENSMUST00000062769 ENSMUST00000114665 ENSMUST00000114668 ENSMUST00000080431 ENSMUST00000006817 ENSMUST00000176653 ENSMUST00000001712 ENSMUST00000049095 ENSMUST00000075675 ENSMUST00000108953 ENSMUST00000175683 ENSMUST00000177211 ENSMUST00000074525 ENSMUST00000162600 ENSMUST00000055758 ENSMUST00000152610 ENSMUST00000112251 ENSMUST00000103181 ENSMUST00000089461 ENSMUST00000090558 ENSMUST00000154782 ENSMUST00000053445 ENSMUST00000109221 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Higd1c
Gene Name:   HIG1 domain family, member 1C
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000093550
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 41.820000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download