PrimePCR™ Probe Assay: Olfr113, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Olfr113, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olfr113, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043761
Assay Design:   exonic
Chromosome Location:   17:37575168-37575273question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000115351 ENSMUST00000122211 ENSMUST00000024805 ENSMUST00000169266 ENSMUST00000033913 ENSMUST00000117243 ENSMUST00000118811 ENSMUST00000095128 ENSMUST00000070084 ENSMUST00000101558 ENSMUST00000115267 ENSMUST00000169130 ENSMUST00000173815 ENSMUST00000166914 ENSMUST00000167899 ENSMUST00000109309 ENSMUST00000039074 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000168047 ENSMUST00000037055 ENSMUST00000127833 ENSMUST00000143431 ENSMUST00000084640 ENSMUST00000033153 ENSMUST00000085548 ENSMUS

Gene Information

Olfactory receptors interact with odorant molecules in the nose to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq Jul 2008]

Gene Symbol:   Olfr113
Gene Name:   Olfactory receptor 113
Aliases:   MOR218-9
RefSeq:   NC_000083.6 NT_039649.8 NT_039666.2 NT_187004.1 NT_187027.1
Ensembl:   ENSMUSG00000092292
Entrez:   258286
Chromosome Mapping:   17 B1|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.060000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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