PrimePCR™ Probe Assay: Mier2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Mier2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Mier2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043751
Assay Design:   exonic
Chromosome Location:   10:79540420-79540513question
Amplicon Length:   64
Splice Variants Targeted:   ENSMUST00000134423 ENSMUST00000093029 ENSMUST00000062855 ENSMUST00000165028 ENSMUST00000172158 ENSMUST00000160583 ENSMUST00000069637 ENSMUST00000078694 ENSMUST00000150762 ENSMUST00000137634 ENSMUST00000087877 ENSMUST00000062967 ENSMUST00000108376 ENSMUST00000109632 ENSMUST00000109636

Gene Information

Description Not Available

Gene Symbol:   Mier2
Gene Name:   Mesoderm induction early response 1, family member 2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000091854
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 39.820000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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