PrimePCR™ Probe Assay: Yy2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043748
Assay Design:   exonic
Chromosome Location:   X:157568264-157568405question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000032396 ENSMUST00000132948 ENSMUST00000112522 ENSMUST00000065806 ENSMUST00000179062 ENSMUST00000172035 ENSMUST00000045840

Gene Information

Description Not Available

Gene Symbol:   Yy2
Gene Name:   Yy2 transcription factor
Aliases:   Not Available
RefSeq:   NC_000086.7 NT_039718.8
Ensembl:   ENSMUSG00000091736
Entrez:   100073351
UniGene:   Not Available
Chromosome Mapping:   X F4|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.160000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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