PrimePCR™ Probe Assay: Vmn2r41, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043725
Assay Design:   exonic
Chromosome Location:   7:8161412-8161562question
Amplicon Length:   121
Splice Variants Targeted:   ENSMUST00000170922 ENSMUST00000169683 ENSMUST00000168807 ENSMUST00000086282 ENSMUST00000146278 ENSMUST00000142934 ENSMUST00000171523 ENSMUST00000094863

Gene Information

Description Not Available

Gene Symbol:   Vmn2r41
Gene Name:   Vomeronasal 2, receptor 41
Aliases:   Not Available
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000090892
Entrez:   100042848
UniGene:   Mm.469892
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 38.760000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download