PrimePCR™ Probe Assay: Rps27, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043720
Assay Design:   exonic
Chromosome Location:   3:90212970-90213209question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000115096 ENSMUST00000073325 ENSMUST00000111088 ENSMUST00000053150 ENSMUST00000046157 ENSMUST00000101099 ENSMUST00000179002 ENSMUST00000178076 ENSMUST00000085079 ENSMUST00000170122

Gene Information

Description Not Available

Gene Symbol:   Rps27
Gene Name:   Ribosomal protein S27
Aliases:   3200001M24Rik
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000090733
Entrez:   57294
UniGene:   Mm.270283
Chromosome Mapping:   3 F1|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 40.100000
Efficiency 85

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download