PrimePCR™ Probe Assay: Rbm12, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043692
Assay Design:   exonic
Chromosome Location:   2:156098017-156098119question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000109604 ENSMUST00000059647 ENSMUST00000131377 ENSMUST00000128499 ENSMUST00000138068 ENSMUST00000067880 ENSMUST00000144537

Gene Information

This gene encodes a protein that contains several RNA-binding motifs potential transmembrane domains and proline-rich regions. This gene and the gene for copine I overlap at map location 2 H2. Two alternatively spliced transcript variants have been identified for this gene. Both variants encode the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   Rbm12
Gene Name:   RNA binding motif protein 12
Aliases:   5730420G12Rik, 9430070C08Rik, AI852903, SWAN, mKIAA0765
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000089824
Entrez:   75710
UniGene:   Mm.27660
Chromosome Mapping:   2|2 H2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 39.800000
Efficiency 84

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download