PrimePCR™ Probe Assay: Rhox3c, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043675
Assay Design:   exonic
Chromosome Location:   X:37470307-37470423question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000115203 ENSMUST00000096459 ENSMUST00000115188 ENSMUST00000123851 ENSMUST00000151387 ENSMUST00000115189 ENSMUST00000115198 ENSMUST00000081327 ENSMUST00000115172 ENSMUST00000096457

Gene Information

Description Not Available

Gene Symbol:   Rhox3c
Gene Name:   Reproductive homeobox 3C
Aliases:   EG665203, Rhox3.3, Rhox3b
RefSeq:   NC_000086.7 NT_187037.1
Ensembl:   ENSMUSG00000079636
Entrez:   100135654
UniGene:   Mm.359489
Chromosome Mapping:   X A3.3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 38.120000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download