PrimePCR™ Probe Assay: Rhox4g, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043673
Assay Design:   exonic
Chromosome Location:   X:37650471-37650566question
Amplicon Length:   66
Splice Variants Targeted:   ENSMUST00000113233 ENSMUST00000046972 ENSMUST00000093162 ENSMUST00000162183 ENSMUST00000043087 ENSMUST00000115194 ENSMUST00000179915 ENSMUST00000096458 ENSMUST00000115183 ENSMUST00000115175 ENSMUST00000089075 ENSMUST00000115170 ENSMUST00000029186 ENSMUST00000109478 ENSMUST00000115166 ENSMUST00000109923 ENSMUST00000021950 ENSMUST00000109921

Gene Information

Description Not Available

Gene Symbol:   Rhox4g
Gene Name:   Reproductive homeobox 4G
Aliases:   Rhox4.6, Rhox4.7, Rhox4f
RefSeq:   NC_000086.7 NT_187037.1
Ensembl:   ENSMUSG00000079628
Entrez:   664608
UniGene:   Not Available
Chromosome Mapping:   X A3.2|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.500000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download