PrimePCR™ Probe Assay: Mef2b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043655
Assay Design:   exonic
Chromosome Location:   8:70167165-70167305question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000058092 ENSMUST00000027940 ENSMUST00000072940 ENSMUST00000093162 ENSMUST00000114571 ENSMUST00000031815 ENSMUST00000077093 ENSMUST00000114572 ENSMUST00000113658 ENSMUST00000102944 ENSMUST00000032279 ENSMUST00000041195 ENSMUST00000036618 ENSMUST00000163756 ENSMUST00000072500 ENSMUST00000164040 ENSMUST00000110146 ENSMUST00000110143 ENSMUST00000110141 ENSMUST00000110140

Gene Information

Description Not Available

Gene Symbol:   Mef2b
Gene Name:   Myocyte enhancer factor 2B
Aliases:   AI451606
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000079033
Entrez:   17259
UniGene:   Mm.432505
Chromosome Mapping:   8 C1|8 33.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 39.090000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download