PrimePCR™ Probe Assay: Aarsd1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043615
Assay Design:   exonic
Chromosome Location:   11:101417382-101417481question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000106763 ENSMUST00000106762 ENSMUST00000057892 ENSMUST00000036315 ENSMUST00000094179 ENSMUST00000151045 ENSMUST00000070395 ENSMUST00000019977

Gene Information

Description Not Available

Gene Symbol:   Aarsd1
Gene Name:   Alanyl-tRNA synthetase domain containing 1
Aliases:   1110069E20Rik, 1700113I22Rik
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000075528
Entrez:   73635
UniGene:   Mm.146283
Chromosome Mapping:   11 D|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.994500
y-intercept 38.600000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download