PrimePCR™ Probe Assay: Bglap, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043580
Assay Design:   exonic
Chromosome Location:   3:88384192-88384433question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000098496 ENSMUST00000025434 ENSMUST00000163367 ENSMUST00000030010 ENSMUST00000160641 ENSMUST00000112068 ENSMUST00000161865 ENSMUST00000112064 ENSMUST00000106237 ENSMUST00000033051 ENSMUST00000177111 ENSMUST00000107543 ENSMUST00000107542 ENSMUST00000075523 ENSMUST00000098956 ENSMUST00000076048 ENSMUST00000116349

Gene Information

Description Not Available

Gene Symbol:   Bglap
Gene Name:   Bone gamma carboxyglutamate protein
Aliases:   Bglap1, Bglap2, OC, OG1, mOC-A
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000074483
Entrez:   12096
UniGene:   Mm.389459
Chromosome Mapping:   3 F1|3 42.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 38.240000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download