PrimePCR™ Probe Assay: Mup14, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043546
Assay Design:   exonic
Chromosome Location:   4:61300132-61300270question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000079697 ENSMUST00000166098 ENSMUST00000074700 ENSMUST00000095058 ENSMUST00000163931 ENSMUST00000122381 ENSMUST00000118759 ENSMUST00000103012 ENSMUST00000098047 ENSMUST00000098046 ENSMUST00000117932 ENSMUST00000072678 ENSMUST00000098042 ENSMUST00000125461 ENSMUST00000075206 ENSMUST00000095049 ENSMUST00000107483 ENSMUST00000107484 ENSMUST00000098040 ENSMUST00000080606 ENSMUST00000086521 ENSMUST00000102490 ENSMUST00000023987 ENSMUST00000091227 ENSMUST00000122174

Gene Information

Description Not Available

Gene Symbol:   Mup14
Gene Name:   Major urinary protein 14
Aliases:   Gm13514, Mup12, OTTMUSG00000012493
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000073830
Entrez:   100039116
UniGene:   Mm.458441
Chromosome Mapping:   4 B3|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 38.630000
Efficiency 83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download