PrimePCR™ Probe Assay: Speer4a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best specificity

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043532
Assay Design:   exonic
Chromosome Location:   5:26034331-26034482question
Amplicon Length:   122
Splice Variants Targeted:   ENSMUST00000168875 ENSMUST00000072286 ENSMUST00000079447 ENSMUST00000095004 ENSMUST00000094946 ENSMUST00000063524 ENSMUST00000088236 ENSMUST00000074148

Gene Information

Description Not Available

Gene Symbol:   Speer4a
Gene Name:   Spermatogenesis associated glutamate (E)-rich protein 4a
Aliases:   1700027N01Rik, SPEER-4A
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000073119
Entrez:   75657
UniGene:   Mm.374273
Chromosome Mapping:   5 B1|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 38.950000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download