PrimePCR™ Probe Assay: Grcc10, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Grcc10, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Grcc10, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043528
Assay Design:   exonic
Chromosome Location:   6:124739302-124739406question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000164107 ENSMUST00000057135 ENSMUST00000028740 ENSMUST00000143051 ENSMUST00000085840 ENSMUST00000169037 ENSMUST00000168545 ENSMUST00000171456 ENSMUST00000098521 ENSMUST00000089684 ENSMUST00000040986 ENSMUST00000115105 ENSMUST00000097425 ENSMUST00000045912 ENSMUST00000029005 ENSMUST00000059472 ENSMUST00000041767 ENSMUST00000004389 ENSMUST00000114109 ENSMUST00000118878 ENSMUST00000101341 ENSMUST00000137823 ENSMUST00000180046 ENSMUST00000101465 ENSMUST00000070402

Gene Information

Description Not Available

Gene Symbol:   Grcc10
Gene Name:   Gene rich cluster, C10 gene
Aliases:   2310033H05Rik, C10
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000072772
Entrez:   14790
Chromosome Mapping:   6 F2|6 60.22 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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