PrimePCR™ Probe Assay: C87414, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043488
Assay Design:   exonic
Chromosome Location:   5:93638812-93671348question
Amplicon Length:   104
Splice Variants Targeted:   ENSMUST00000179700 ENSMUST00000162964 ENSMUST00000159489 ENSMUST00000101024 ENSMUST00000076321 ENSMUST00000162539 ENSMUST00000159578 ENSMUST00000160382 ENSMUST00000160418 ENSMUST00000097477 ENSMUST00000178646 ENSMUST00000101015 ENSMUST00000160045 ENSMUST00000094593 ENSMUST00000171752 ENSMUST00000071182

Gene Information

Description Not Available

Gene Symbol:   C87414
Gene Name:   Expressed sequence C87414
Aliases:   Not Available
RefSeq:   NC_000071.6 NT_109320.5
Ensembl:   ENSMUSG00000070686
Entrez:   381654
UniGene:   Mm.326591
Chromosome Mapping:   5 E2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.860000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download