PrimePCR™ Probe Assay: Gm10159, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gm10159, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gm10159, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043477
Assay Design:   exonic
Chromosome Location:   7:88530424-88530558question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000089950 ENSMUST00000170953 ENSMUST00000113360 ENSMUST00000097666 ENSMUST00000127504 ENSMUST00000179929 ENSMUST00000038096 ENSMUST00000179598 ENSMUST00000030391 ENSMUST00000099058 ENSMUST00000170744 ENSMUST00000107383 ENSMUST00000032754 ENSMUST00000093344 ENSMUST00000111067 ENSMUST00000067124 ENSMUST00000053918 ENSMUST00000040853

Gene Information

Description Not Available

Gene Symbol:   Gm10159
Gene Name:   Predicted pseudogene 10159
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000069972
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 38.350000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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