PrimePCR™ Probe Assay: Hist1h4b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043457
Assay Design:   exonic
Chromosome Location:   13:23757024-23757184question
Amplicon Length:   131
Splice Variants Targeted:   ENSMUST00000055993 ENSMUST00000120876 ENSMUST00000069044 ENSMUST00000079618 ENSMUST00000075290 ENSMUST00000087714 ENSMUST00000102983 ENSMUST00000104941 ENSMUST00000102979 ENSMUST00000102977 ENSMUST00000102972 ENSMUST00000102971 ENSMUST00000102967 ENSMUST00000102965 ENSMUST00000102964 ENSMUST00000009138 ENSMUST00000119274 ENSMUST00000061835 ENSMUST00000177086 ENSMUST00000078259 ENSMUST00000089217 ENSMUST00000001989 ENSMUST00000039734 ENSMUST00000160896 ENSMUST00000015271 ENSMUST00000023068 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist1h4b
Gene Name:   Histone cluster 1, H4b
Aliases:   Not Available
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000069266
Entrez:   326620
UniGene:   Mm.261642
Chromosome Mapping:   13 A2-A3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.070000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download