PrimePCR™ Probe Assay: Hist2h2ac, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hist2h2ac, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hist2h2ac, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043451
Assay Design:   exonic
Chromosome Location:   3:96220651-96220854question
Amplicon Length:   174
Splice Variants Targeted:   ENSMUST00000092415 ENSMUST00000108709 ENSMUST00000070124 ENSMUST00000074752 ENSMUST00000091751 ENSMUST00000091745 ENSMUST00000081342 ENSMUST00000091742 ENSMUST00000091741 ENSMUST00000073261 ENSMUST00000102969 ENSMUST00000090776 ENSMUST00000171127 ENSMUST00000078369 ENSMUST00000019183 ENSMUST00000070203 ENSMUST00000127825 ENSMUST00000151288 ENSMUST00000073115 ENSMUST00000090782 ENSMUST00000078756 ENSMUST00000074976 ENSMUST00000037551 ENSMUST00000159294 ENSMUST00000177755

Gene Information

Description Not Available

Gene Symbol:   Hist2h2ac
Gene Name:   Histone cluster 2, H2ac
Aliases:   H2a-613b
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000068855
Entrez:   319176
Chromosome Mapping:   3 F1-F2|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 39.160000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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