PrimePCR™ Probe Assay: Spr-ps1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Spr-ps1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Spr-ps1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043444
Assay Design:   exonic
Chromosome Location:   6:85155085-85155175question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000170430 ENSMUST00000170651 ENSMUST00000025234 ENSMUST00000034365 ENSMUST00000149582 ENSMUST00000134469 ENSMUST00000109579 ENSMUST00000131235 ENSMUST00000135047 ENSMUST00000107779 ENSMUST00000003961 ENSMUST00000106450 ENSMUST00000174822 ENSMUST00000106451 ENSMUST00000075036 ENSMUST00000108684 ENSMUST00000180845 ENSMUST00000081911 ENSMUST00000051765 ENSMUST00000020003 ENSMUST00000163761 ENSMUST00000021368 ENSMUST00000045986 ENSMUST00000174769 ENSMUST00000174286 ENSMUST00000089584

Gene Information

Description Not Available

Gene Symbol:   Spr-ps1
Gene Name:   Sepiapterin reductase pseudogene 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000068303
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.830000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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