PrimePCR™ Probe Assay: Hist1h2bh, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hist1h2bh, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hist1h2bh, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043393
Assay Design:   exonic
Chromosome Location:   13:23543139-23543259question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000152080 ENSMUST00000091756 ENSMUST00000110476 ENSMUST00000091709 ENSMUST00000110473 ENSMUST00000102982 ENSMUST00000110469 ENSMUST00000091749 ENSMUST00000110467 ENSMUST00000180288 ENSMUST00000110455 ENSMUST00000110452 ENSMUST00000078156 ENSMUST00000079251 ENSMUST00000105106 ENSMUST00000091704 ENSMUST00000051091 ENSMUST00000018246 ENSMUST00000099703 ENSMUST00000052776 ENSMUST00000126070 ENSMUST00000013773 ENSMUST00000174367 ENSMUST00000170329 ENSMUST00000031049 ENSMUST00000103069 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist1h2bh
Gene Name:   Histone cluster 1, H2bh
Aliases:   H2b-221
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000064168
Entrez:   319182
Chromosome Mapping:   13 A2-A3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.610000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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