PrimePCR™ Probe Assay: Fbln2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043391
Assay Design:   exonic
Chromosome Location:   6:91233117-91233229question
Amplicon Length:   83
Splice Variants Targeted:   ENSMUST00000051310 ENSMUST00000087320 ENSMUST00000037992 ENSMUST00000113852 ENSMUST00000095527 ENSMUST00000021290 ENSMUST00000113498 ENSMUST00000041544 ENSMUST00000132021 ENSMUST00000134974 ENSMUST00000048455 ENSMUST00000102873 ENSMUST00000091153 ENSMUST00000071201 ENSMUST00000177689 ENSMUST00000152008

Gene Information

Description Not Available

Gene Symbol:   Fbln2
Gene Name:   Fibulin 2
Aliases:   5730577E14Rik
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000064080
Entrez:   14115
UniGene:   Mm.249146
Chromosome Mapping:   6 D|6 37.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.180000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download