PrimePCR™ Probe Assay: Hist1h3d, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hist1h3d, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hist1h3d, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043368
Assay Design:   exonic
Chromosome Location:   13:23576123-23576216question
Amplicon Length:   64
Splice Variants Targeted:   ENSMUST00000128505 ENSMUST00000075845 ENSMUST00000171937 ENSMUST00000091754 ENSMUST00000099704 ENSMUST00000080859 ENSMUST00000075558 ENSMUST00000105107 ENSMUST00000105105 ENSMUST00000091752 ENSMUST00000091703 ENSMUST00000091701 ENSMUST00000013693 ENSMUST00000054287 ENSMUST00000042290 ENSMUST00000037615 ENSMUST00000161308 ENSMUST00000081026 ENSMUST00000162814 ENSMUST00000025500 ENSMUST00000167199 ENSMUST00000059295 ENSMUST00000163785 ENSMUST00000073724 ENSMUST00000041415 ENSMUST00000034304 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist1h3d
Gene Name:   Histone cluster 1, H3d
Aliases:   H3-b
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000062808
Entrez:   319149
Chromosome Mapping:   13 A2-A3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.850000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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