PrimePCR™ Probe Assay: Rpl7a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043361
Assay Design:   exonic
Chromosome Location:   2:26911422-26911566question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000049113 ENSMUST00000166650 ENSMUST00000096427 ENSMUST00000094754 ENSMUST00000027768 ENSMUST00000071565 ENSMUST00000104977 ENSMUST00000095218 ENSMUST00000177716 ENSMUST00000071172 ENSMUST00000090170 ENSMUST00000036576 ENSMUST00000177657 ENSMUST00000102898 ENSMUST00000033818 ENSMUST00000091237 ENSMUST00000132974 ENSMUST00000085365 ENSMUST00000171235

Gene Information

Description Not Available

Gene Symbol:   Rpl7a
Gene Name:   Ribosomal protein L7A
Aliases:   Surf3
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000062647
Entrez:   27176
UniGene:   Mm.398371
Chromosome Mapping:   2 A3|2 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download