PrimePCR™ Probe Assay: Mageb1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043347
Assay Design:   exonic
Chromosome Location:   X:92015470-92015572question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000114013 ENSMUST00000145419 ENSMUST00000041782 ENSMUST00000167020 ENSMUST00000092112 ENSMUST00000099471 ENSMUST00000072269 ENSMUST00000063607 ENSMUST00000126792 ENSMUST00000106549 ENSMUST00000106550 ENSMUST00000168529 ENSMUST00000174724 ENSMUST00000173126 ENSMUST00000100750 ENSMUST00000170481 ENSMUST00000154132 ENSMUST00000104936 ENSMUST00000101397 ENSMUST00000063726 ENSMUST00000101396

Gene Information

Description Not Available

Gene Symbol:   Mageb1
Gene Name:   Melanoma antigen, family B, 1
Aliases:   MAGE-Xp, Mage-b1, Mage-rs1, Magel1, Smage1, dam1
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000062162
Entrez:   17145
UniGene:   Mm.377100
Chromosome Mapping:   X C2|X 35.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 37.360000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download