PrimePCR™ Probe Assay: Defb35, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Defb35, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Defb35, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043262
Assay Design:   exonic
Chromosome Location:   8:21940734-21940838question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000020999 ENSMUST00000062113 ENSMUST00000085894 ENSMUST00000161017 ENSMUST00000160789 ENSMUST00000076786 ENSMUST00000101617 ENSMUST00000065090 ENSMUST00000090474 ENSMUST00000028499 ENSMUST00000111740 ENSMUST00000049519

Gene Information

Description Not Available

Gene Symbol:   Defb35
Gene Name:   Defensin beta 35
Aliases:   BD-35
RefSeq:   NC_000074.6 NT_039457.8
Ensembl:   ENSMUSG00000058052
Entrez:   246084
Chromosome Mapping:   8 A2|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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