PrimePCR™ Probe Assay: Defb34, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043205
Assay Design:   exonic
Chromosome Location:   8:19126406-19126501question
Amplicon Length:   66
Splice Variants Targeted:   ENSMUST00000064475 ENSMUST00000062586 ENSMUST00000079961 ENSMUST00000109547 ENSMUST00000036372 ENSMUST00000159880 ENSMUST00000025831 ENSMUST00000167427 ENSMUST00000003310 ENSMUST00000150042 ENSMUST00000064334 ENSMUST00000072993 ENSMUST00000060077

Gene Information

Description Not Available

Gene Symbol:   Defb34
Gene Name:   Defensin beta 34
Aliases:   BD-34
RefSeq:   NC_000074.6 NT_039455.8
Ensembl:   ENSMUSG00000052554
Entrez:   360211
UniGene:   Mm.271557
Chromosome Mapping:   8 A1.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.660000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download