PrimePCR™ Probe Assay: Hist2h2bb, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043196
Assay Design:   exonic
Chromosome Location:   3:96269950-96270095question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000091756 ENSMUST00000110476 ENSMUST00000091709 ENSMUST00000110473 ENSMUST00000102982 ENSMUST00000110469 ENSMUST00000091749 ENSMUST00000110467 ENSMUST00000180288 ENSMUST00000110455 ENSMUST00000110452 ENSMUST00000078156 ENSMUST00000079251 ENSMUST00000105106 ENSMUST00000091704 ENSMUST00000051091 ENSMUST00000018246 ENSMUST00000099703 ENSMUST00000052776 ENSMUST00000027587 ENSMUST00000025338 ENSMUST00000051674 ENSMUST00000177113 ENSMUST00000090559

Gene Information

Description Not Available

Gene Symbol:   Hist2h2bb
Gene Name:   Histone cluster 2, H2bb
Aliases:   H2b-616
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000050936
Entrez:   319189
UniGene:   Mm.451071
Chromosome Mapping:   3 F1-F2|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download