PrimePCR™ Probe Assay: Duxbl, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043183
Assay Design:   exonic
Chromosome Location:   14:25984156-25984271question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000062317 ENSMUST00000101141 ENSMUST00000172667 ENSMUST00000173580 ENSMUST00000173155 ENSMUST00000049793 ENSMUST00000172038 ENSMUST00000174564 ENSMUST00000173617 ENSMUST00000100810 ENSMUST00000112340 ENSMUST00000172517 ENSMUST00000174494 ENSMUST00000100804 ENSMUST00000025680

Gene Information

Description Not Available

Gene Symbol:   Duxbl
Gene Name:   Double homeobox B-like
Aliases:   1110051B16Rik, Duxl
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000048502
Entrez:   278672
UniGene:   Mm.440101
Chromosome Mapping:   14 A3|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download