PrimePCR™ Probe Assay: Zfp738, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043181
Assay Design:   exonic
Chromosome Location:   13:67673578-67673674question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000091481 ENSMUST00000167565 ENSMUST00000091526 ENSMUST00000021997 ENSMUST00000120861 ENSMUST00000045969 ENSMUST00000049705 ENSMUST00000172266 ENSMUST00000057070 ENSMUST00000181071 ENSMUST00000109732 ENSMUST00000056470 ENSMUST00000057241 ENSMUST00000075255 ENSMUST00000019572 ENSMUST00000171518 ENSMUST00000076123 ENSMUST00000091523 ENSMUST00000163534 ENSMUST00000181767 ENSMUST00000181341 ENSMUST00000181573 ENSMUST00000181892 ENSMUST00000053289 ENSMUST00000012873 ENSMUST00000138725 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Zfp738
Gene Name:   Zinc finger protein 738
Aliases:   3830402I07Rik, 6720487G11Rik
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000048280
Entrez:   408068
UniGene:   Mm.435551
Chromosome Mapping:   13 B3|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.820000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download