PrimePCR™ Probe Assay: Hmga1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043172
Assay Design:   exonic
Chromosome Location:   17:27562773-27562910question
Amplicon Length:   108
Splice Variants Targeted:   ENSMUST00000117600 ENSMUST00000114888 ENSMUST00000119486 ENSMUST00000117254 ENSMUST00000118570 ENSMUST00000079869 ENSMUST00000105046 ENSMUST00000070174 ENSMUST00000174697 ENSMUST00000174072 ENSMUST00000056660 ENSMUST00000119810 ENSMUST00000032968 ENSMUST00000161028 ENSMUST00000089362 ENSMUST00000089360

Gene Information

This locus encodes a member of the nuclear non-histone high mobility group protein family. This architectural transcription factor binds to A-T rich DNA sequences and participates in enhanceosome formation chromatin remodeling and regulation of transcription. This protein functions in many cellular processes including cell growth and differentiation. Alternatively spliced transcript variants have been described. [provided by RefSeq Oct 2009]

Gene Symbol:   Hmga1
Gene Name:   High mobility group AT-hook 1
Aliases:   AL023995, Hmga1a, Hmga1b, Hmgi, Hmgiy, Hmgy
RefSeq:   NC_000083.6 NT_039649.8 NT_187005.1
Ensembl:   ENSMUSG00000046711
Entrez:   15361
UniGene:   Mm.4438
Chromosome Mapping:   17 A3.3|17 13.19 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.770000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download