PrimePCR™ Probe Assay: Alkbh4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043135
Assay Design:   exonic
Chromosome Location:   5:136140402-136140506question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000034448 ENSMUST00000058126 ENSMUST00000105297 ENSMUST00000105296 ENSMUST00000040990 ENSMUST00000057910 ENSMUST00000041415 ENSMUST00000033088 ENSMUST00000136634 ENSMUST00000041100 ENSMUST00000143229 ENSMUST00000100568 ENSMUST00000148750 ENSMUST00000113218 ENSMUST00000156238 ENSMUST00000144713 ENSMUST00000053498 ENSMUST00000069623

Gene Information

Description Not Available

Gene Symbol:   Alkbh4
Gene Name:   AlkB, alkylation repair homolog 4 (E. coli)
Aliases:   2010004B12Rik, Abj4
RefSeq:   NC_000071.6 NT_039314.8
Ensembl:   ENSMUSG00000039754
Entrez:   72041
UniGene:   Mm.486605
Chromosome Mapping:   5|5 G1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.800000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download