PrimePCR™ Probe Assay: Rbm42, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best specificity

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rbm42, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rbm42, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043118
Assay Design:   exonic
Chromosome Location:   7:30644847-30644967question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000039390 ENSMUST00000003038 ENSMUST00000119292 ENSMUST00000025751 ENSMUST00000101618 ENSMUST00000042726 ENSMUST00000108141 ENSMUST00000038757 ENSMUST00000022623 ENSMUST00000096243 ENSMUST00000020441 ENSMUST00000036579 ENSMUST00000070203 ENSMUST00000127825 ENSMUST00000118408 ENSMUST00000119077 ENSMUST00000094148 ENSMUST00000084238 ENSMUST00000178945 ENSMUST00000171634 ENSMUST00000073418 ENSMUST00000165738 ENSMUST00000075689 ENSMUST00000057407 ENSMUST00000113334 ENSMUST00000048964 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rbm42
Gene Name:   RNA binding motif protein 42
Aliases:   1700003D06Rik, 3100004P22Rik
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000036733
Entrez:   68035
Chromosome Mapping:   7 B1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.040000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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