PrimePCR™ Probe Assay: Mobp, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043101
Assay Design:   exonic
Chromosome Location:   9:120167882-120167976question
Amplicon Length:   65
Splice Variants Targeted:   ENSMUST00000113800 ENSMUST00000099497 ENSMUST00000019276 ENSMUST00000023805 ENSMUST00000120122 ENSMUST00000034529 ENSMUST00000166367 ENSMUST00000042485 ENSMUST00000119722 ENSMUST00000143031 ENSMUST00000044217 ENSMUST00000033611 ENSMUST00000034090 ENSMUST00000084086 ENSMUST00000090190 ENSMUST00000115698 ENSMUST00000006053 ENSMUST00000163476 ENSMUST00000165363 ENSMUST00000090159 ENSMUST00000170996 ENSMUST00000170366 ENSMUST00000163997 ENSMUST00000170273 ENSMUST00000108408 ENSMUST00000112986 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mobp
Gene Name:   Myelin-associated oligodendrocytic basic protein
Aliases:   AI835053, AI839848, MOBP155, MOBP170, MOBP69, MOBP73, MOBP81, R74645
RefSeq:   NC_000075.6 NT_039482.8
Ensembl:   ENSMUSG00000032517
Entrez:   17433
UniGene:   Mm.486167
Chromosome Mapping:   9 F4|9 69.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.860000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download