PrimePCR™ Probe Assay: Dnaja1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043076
Assay Design:   exonic
Chromosome Location:   4:40724021-40724139question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000108283 ENSMUST00000032823 ENSMUST00000120452 ENSMUST00000154690 ENSMUST00000070070 ENSMUST00000030118 ENSMUST00000137246 ENSMUST00000125442 ENSMUST00000149794 ENSMUST00000164233 ENSMUST00000048155 ENSMUST00000079987 ENSMUST00000113864

Gene Information

Description Not Available

Gene Symbol:   Dnaja1
Gene Name:   DnaJ (Hsp40) homolog, subfamily A, member 1
Aliases:   Hsj2, Nedd7
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000028410
Entrez:   15502
UniGene:   Mm.27897
Chromosome Mapping:   4 A5|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.490000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download