PrimePCR™ Probe Assay: Tpm3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best coverage

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043073
Assay Design:   exonic
Chromosome Location:   3:90090010-90099549question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000003575 ENSMUST00000072359 ENSMUST00000057634 ENSMUST00000167887 ENSMUST00000030184 ENSMUST00000107914 ENSMUST00000107913 ENSMUST00000053734 ENSMUST00000121503 ENSMUST00000119570 ENSMUST00000029549 ENSMUST00000118566 ENSMUST00000119158 ENSMUST00000062193

Gene Information

Description Not Available

Gene Symbol:   Tpm3
Gene Name:   Tropomyosin 3, gamma
Aliases:   Tm5NM, Tpm-5, Tpm5, Trop-5, gamma-TM, hTM30nm, hTMnm
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000027940
Entrez:   59069
UniGene:   Mm.240839
Chromosome Mapping:   3 F1|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 41.170000
Efficiency 81

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download