PrimePCR™ Probe Assay: Speg, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043060
Assay Design:   exonic
Chromosome Location:   1:75402357-75402456question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000087122 ENSMUST00000113590 ENSMUST00000113589 ENSMUST00000113588 ENSMUST00000113587 ENSMUST00000122266 ENSMUST00000039752 ENSMUST00000122058 ENSMUST00000025020 ENSMUST00000067663 ENSMUST00000002171 ENSMUST00000114299 ENSMUST00000033699 ENSMUST00000130007 ENSMUST00000101454 ENSMUST00000015645 ENSMUST00000021331 ENSMUST00000056977 ENSMUST00000053498 ENSMUST00000167258 ENSMUST00000107680

Gene Information

This gene encodes a protein with similarity to members of the myosin light chain kinase family. This protein family is required for myocyte cytoskeletal development. Studies have determined that a lack of this protein affected myocardial development. Multiple alternatively spliced transcript variants that encode different protein isoforms have been defined. [provided by RefSeq Mar 2010]

Gene Symbol:   Speg
Gene Name:   SPEG complex locus
Aliases:   AW125581, Apeg1, BPEG, D1Bwg1450e, SPEGalpha, SPEGbeta, mKIAA1297
RefSeq:   NC_000067.6 NT_039173.8
Ensembl:   ENSMUSG00000026207
Entrez:   11790
UniGene:   Mm.275397
Chromosome Mapping:   1 C4|1 41.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.570000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download