PrimePCR™ Probe Assay: Cabp2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043047
Assay Design:   exonic
Chromosome Location:   19:4087118-4087262question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000106739 ENSMUST00000106741 ENSMUST00000033339 ENSMUST00000038743 ENSMUST00000121743 ENSMUST00000165147 ENSMUST00000027528 ENSMUST00000122865 ENSMUST00000056915 ENSMUST00000089293 ENSMUST00000159148 ENSMUST00000159556 ENSMUST00000162908 ENSMUST00000042750 ENSMUST00000088561 ENSMUST00000118234 ENSMUST00000120066 ENSMUST00000088786 ENSMUST00000095171 ENSMUST00000109486 ENSMUST00000109485 ENSMUST00000141497 ENSMUST00000046274 ENSMUST00000109155 ENSMUST00000063710 ENSMUST00000109930 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Cabp2
Gene Name:   Calcium binding protein 2
Aliases:   Not Available
RefSeq:   NC_000085.6 NT_082868.6 NW_004058054.1
Ensembl:   ENSMUSG00000024857
Entrez:   29866
UniGene:   Mm.46268
Chromosome Mapping:   19 A|19

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.980000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download