PrimePCR™ Probe Assay: Cdc34-ps, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Cdc34-ps, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cdc34-ps, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043028
Assay Design:   exonic
Chromosome Location:   11:94741794-94741917question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000040455 ENSMUST00000005532 ENSMUST00000117419 ENSMUST00000085913 ENSMUST00000034619 ENSMUST00000040789 ENSMUST00000064462 ENSMUST00000059193 ENSMUST00000107658 ENSMUST00000107657 ENSMUST00000081775 ENSMUST00000167149 ENSMUST00000167110 ENSMUST00000169828 ENSMUST00000163168 ENSMUST00000167487 ENSMUST00000046233 ENSMUST00000022535 ENSMUST00000042970 ENSMUST00000060435 ENSMUST00000084215 ENSMUST00000105831 ENSMUST00000084214 ENSMUST00000021240 ENSMUST00000146385

Gene Information

Description Not Available

Gene Symbol:   Cdc34-ps
Gene Name:   Cell division cycle 34 homolog, pseudogene (S. cerevisiae)
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000020870
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 38.750000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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