PrimePCR™ Probe Assay: Hbs1l, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043023
Assay Design:   exonic
Chromosome Location:   10:21307693-21336975question
Amplicon Length:   65
Splice Variants Targeted:   ENSMUST00000004920 ENSMUST00000179209 ENSMUST00000078755 ENSMUST00000091967 ENSMUST00000025239 ENSMUST00000061324 ENSMUST00000096200 ENSMUST00000020153 ENSMUST00000092674 ENSMUST00000028633 ENSMUST00000103234 ENSMUST00000017620 ENSMUST00000107783 ENSMUST00000107782 ENSMUST00000030011 ENSMUST00000102748 ENSMUST00000102747 ENSMUST00000067542

Gene Information

Description Not Available

Gene Symbol:   Hbs1l
Gene Name:   Hbs1-like (S. cerevisiae)
Aliases:   2810035F15Rik, AI326327, eRFS
RefSeq:   NC_000076.6 NT_039492.8
Ensembl:   ENSMUSG00000019977
Entrez:   56422
UniGene:   Mm.25527
Chromosome Mapping:   10 A3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 38.230000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download