PrimePCR™ Probe Assay: Lim2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Lim2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Lim2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043004
Assay Design:   exonic
Chromosome Location:   7:43430640-43430731question
Amplicon Length:   62
Splice Variants Targeted:   ENSMUST00000084727 ENSMUST00000169638 ENSMUST00000080718 ENSMUST00000031967 ENSMUST00000161178 ENSMUST00000050349 ENSMUST00000004732 ENSMUST00000167626 ENSMUST00000026826 ENSMUST00000179998 ENSMUST00000040153 ENSMUST00000112832 ENSMUST00000178486 ENSMUST00000023741 ENSMUST00000028995 ENSMUST00000063063 ENSMUST00000105494 ENSMUST00000042470 ENSMUST00000047075 ENSMUST00000047157 ENSMUST00000114159 ENSMUST00000028300 ENSMUST00000108375 ENSMUST00000108376 ENSMUST00000102488 ENSMUST00000168348 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Lim2
Gene Name:   Lens intrinsic membrane protein 2
Aliases:   19kDa, 4833403J20, MP19, To3
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000004613
Entrez:   233187
Chromosome Mapping:   7 B4|7 2.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 38.150000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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