PrimePCR™ Probe Assay: Nfe2l1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042588
Assay Design:   exonic
Chromosome Location:   11:96822114-96822244question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000107658 ENSMUST00000107659 ENSMUST00000107657 ENSMUST00000126949 ENSMUST00000142065 ENSMUST00000081775 ENSMUST00000167149 ENSMUST00000167110 ENSMUST00000169828

Gene Information

This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases from the use of NRF1 for this gene NFE2L1 and for "nuclear respiratory factor 1" which has an official symbol of NRF1. [provided by RefSeq Jul 2008]

Gene Symbol:   Nfe2l1
Gene Name:   Nuclear factor, erythroid derived 2,-like 1
Aliases:   AA408798, AW212678, LCR-F1, Lcrf1, NRF1, TCF-11, TCF11
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000038615
Entrez:   18023
UniGene:   Mm.6743
Chromosome Mapping:   11 D-E|11 57.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download